產品詳情
胰蛋白酶（Trypsin）是一種絲氨酸水解酶，它能夠把多肽鏈中賴氨酸和精氨酸殘基中的羥基側切段，水解細胞間的蛋白質，破壞細胞間的連接，從而使組織或貼壁細胞離散成單個細胞。胰酶分散細胞的活性與組織或細胞的特性、胰酶濃度、溫度和作用時間有關，在pH8.0和37°C時，胰酶的作用能力最強。因此，在使用胰酶時，應當把握好濃度、溫度和時間，以免消化過度造成細胞損傷。一般常用胰酶的工作濃度為0.25%，而半貼壁細胞或對胰酶敏感的細胞常采用低濃度（0.05%）的胰酶進行細胞消化。由于EDTA能夠螯合Ca2+和Mg2+，從而破壞細胞連接促進細胞的解離，因此在胰酶溶液中常常會加入一定量的EDTA混合使用，以增強解離效果。對于EDTA可能會干擾后續的測試分析時，推薦選擇不含EDTA的消化液。具體請參考以下表格選擇合適的胰蛋白酶產品。
儲存：-20度，干冰運輸
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